In the most simple form of an elisa, antigens from the sample to be tested are attached to a surface. Learn about the different methods for performing an elisa assay for protein quantitation, including assay design strategies and reagents. Elisa is a laboratory technique that detects certain antibodies, antigens and other substances in your blood, pee or other bodily fluid Laboratory scientists use this technique for several medical tests — from diagnosing infections to confirming pregnancy. It is a method of quantifying an antigen immobilized on a solid surface. This guide explains what an elisa (short for enzyme linked immunosorbent assay) is, the procedures involved, types of elisa, detection options and results
What does elisa detect and measure An elisa is an indirect measure of the binding of antibody with antigen The quality of the assay will depend in large part on the specificity of the antibody for the antigen If the specificity is poor, there will be a highly nonspecific background. Elisa tests can detect hormones, bacterial antigens, and antibodies Read on to learn how the elisa test works and how it's used.
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