In the most simple form of an elisa, antigens from the sample to be tested are attached to a surface. Learn about the different methods for performing an elisa assay for protein quantitation, including assay design strategies and reagents. Elisa is a laboratory technique that detects certain antibodies, antigens and other substances in your blood, pee or other bodily fluid Laboratory scientists use this technique for several medical tests — from diagnosing infections to confirming pregnancy. It is a method of quantifying an antigen immobilized on a solid surface.
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