Applications the megascript t7 kit is intended for the synthesis of large amounts of unlabeled or low specific activity rna for a variety of uses, including in vitro translation, antisense/microinjection studies, and isolation of rna binding proteins. T7 rna synthesis kit our t7 rna synthesis kit is designed to provide flexible, high yield synthesis of rna using in vitro transcription with t7 rna polymerase The synthesized rna can be used for downstream applications such as rna structure and function studies, microinjection, transfection, in situ hybridization and in vitro translation. Durascript rna has demonstrated advantages for rna aptamer synthesis, and ribozyme, rna interference (rnai) and antisense rna studies Features durascript rna is resistant to rnase a and dnase high yields of durascript rna t7 in vitro transcription reactions Durascribe t7 rna polymerase uses the same t7 promoters as the wild type t7 rna.
In vitro transcription products include rna synthesis systems, nuclear extracts, ribonucleotides, cloning vectors and supporting products for transcription, primer extension and gene expression studies. Takara ivtpro t7 mrna synthesis kit (cat # 6144 or 6134)—includes a highly efficient t7 rna polymerase mix optimized for preparing high yields of mrna through in vitro transcription The kit also allows integration with capping systems (not included) and rna modification with modified ntps. The ambion™ megascript™ rnai kit is for the synthesis of large mass amounts of dsrna for rnai experiments in nonmammalian systems The kit includes sufficient reagents for 20 reactions.
