These tasks typically require expertise, the use of multiple software tools, and several disjointed steps For typical flow cytometry assays, specimens are processed separately with the antibody panels to enumerate lymphocyte subsets of interest, yielding a sample by sample processing for each individual being tested Although several hundred samples can be processed using this approach, what if there were methods to optimize efficiency of the flow cytometry testing. Fluorescent analyzers and sorters with up to seven lasers and the potential to detect up to 50 parameters are changing the way flow cytometry is used, but old school practices which are inadequate for new technologies remain alive This chapter summarizes recent advances. Cellprofiler can be used to analyze the resulting images from imaging flow cytometry, whether brightfield, darkfield, or fluorescence
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